Metrics

PyFibre calculates properties for the global images and each segmented region. The resultant databases for each section are then labelled:

``{directory}/data/{prefix}_global.h5`` = global image output (also in .xls format)
``{directory}/data/{prefix}_fibre.h5`` = fibre segmented image output (also in .xls format)
``{directory}/data/{prefix}_network.h5`` = fibre networks output (also in .xls format)
``{directory}/data/{prefix}_cell.h5`` = cell segmented image output (also in .xls format)

The database metrics have the following columns:

Table 2 SHG-PL-Trans Image Metrics

Metric

Description

Category

No. Fibres

Number of extracted fibres

Content

Fibre Waviness

Average fibre waviness (length / displacement)

Content

Fibre Lengths

Average fibre pixel length

Content

Fibre Angles

Average fibre angle

Content

Fibre Segment Coverage

Ratio of image containing fibres

Content

Fibre Segment Area

Area of image (in pixels) containing fibres

Shape

Fibre Segment Linearity

Average ratio of diameter to perimeter for fibre segments

Shape

Fibre Segment Eccentricity

Average eccentricity metric of fibre segments

Shape

Fibre Segment SHG Angle SDI

Angle spectrum SDI (mean / max) for all SHG image pixels in fibre regions

Texture

Fibre Segment SHG Anisotropy

Anisotropy of structure tensor for all SHG image pixels in fibre regions

Texture

Fibre Segment SHG Pixel Anisotropy

Mean anisotropy of structure tensor for all SHG image pixels in fibre regions

Texture

Fibre Segment SHG Mean

Mean pixel intensity of SHG image in fibre segment

Texture

Fibre Segment SHG STD

Standard deviation of pixel intensity of SHG image in fibre segment

Texture

Fibre Segment SHG Entropy

Average Shannon entropy of pixel intensities of SHG image in fibre segment

Texture

Fibre Network Degree

Average fibre network number of edges per node

Network

Fibre Network Eigenvalue

Max eigenvalue of network adjacency matrix

Network

Fibre Network Connectivity

Average fibre network connectivity

Network

Fibre Network Cross-Link Density

Average cross-links per fibre

Content

No. Cells

Number of cell segments

Content

Cell Segment Coverage

Ratio of image containing fibres/cells

Content

Cell Segment Area

Area of image (in pixels) containing cells

Shape

Cell Segment Linearity

Average ratio of diameter to perimeter for cell segments

Shape

Cell Segment Eccentricity

Average eccentricity metric of cell segments

Shape

Cell Segment PL Angle SDI

Angle spectrum SDI (mean / max) for all PL image pixels in cell regions

Texture

Cell Segment PL Anisotropy

Anisotropy of structure tensor for all PL image pixels in cell regions

Texture

Cell Segment PL Pixel Anisotropy

Mean anisotropy of structure tensor for all PL image pixels in cell regions

Texture

Cell Segment PL Intensity Mean

Mean pixel intensity of PL image in cell segment

Texture

Cell Segment PL Intensity STD

Standard deviation of pixel intensity of PL image in cell segment

Texture

Cell Segment PL Intensity Entropy

Average Shannon entropy of pixel intensities of PL image in cell segment

Texture